Journal
FOOD AND BIOPRODUCTS PROCESSING
Volume 115, Issue -, Pages 68-77Publisher
INST CHEMICAL ENGINEERS
DOI: 10.1016/j.fbp.2019.02.002
Keywords
Lipases; Yarrowia lipolytica; mango; agroindustrial residues; nitrogen; fermented solid; biocatalyst
Funding
- Carlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro
- Coordination for the Improvement of Higher Level -or Education Personnel
- National Council for Scientific and Technological Development
- FAPERJ (Brazil) [E-26/202.870/2015 BOLSA]
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Mango seed and peel, wastes from the industrial processing of the fruit, were evaluated for lipase production by Yarrowia lipolytica. Submerged fermentation of the components of mango wastes (peel, tegument and kernel) were performed with the components separately and their combinations without any supplementation. The tegument was the only part that showed promising results for lipase production and, therefore several nitrogen sources were used, separately, as supplementation. Yeast extract was the best nitrogen source for lipase production with mango tegument in submerged fermentation, achieving around 3500 U/L of extracellular lipase. Optimum conditions for lipase production were pH 5.0; 187 rpm of agitation speed; temperature of 27.9 degrees C and inoculum concentration of 0.96 g/L. Lipase activity of the solid residue obtained after fermentation carried out in the best experimental conditions was 68.03 U/(g of residue) evidencing that the lipase produced during fermentation adsorbed in the fermented solid, producing a new biocatalyst. (C) 2019 Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
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