Transcriptome analysis provides insights into molecular immune mechanisms of rabbitfish, Siganus oramin against Cryptocaryon irritans infection

The rabbitfish Sigma oramin is resistant to the ciliate parasite Cryptocaryon irritans. L-amino acid oxidase (LAAO) protein from rabbitfish can kill C. irritans in vitro, however, other immune defence mechanisms against C. irritans remains unknown. Here, we generated transcriptomes of rabbitfish skin at 12 h post infection (PI) by C. irritans. The transcriptomes contained 238, 504, 124 clean reads were obtained and then assembled into 258,869 unigenes with an average length of 621 bp and an N50 of 833 bp. Among them, we obtained 418 differentially expressed genes (DEGs) in the skin of rabbitfish wider C. irritans infection and control conditions, including 336 significantly up-regulated genes and 82 significantly down-regulated genes. Seven immune-related categories with 32 differentially expressed immune genes were obtained using Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. DEGs included innate immune molecules, such as LAAO, antimicrobial peptide, lysozyme g, as well as complement components, chemokines and chemokine receptors, NOD-like receptor/Toll-like receptor signaling pathway molecules, antigen processing and T/B cell activation and proliferation molecules. We further validated the expression results of nine immune-related DEGs using quantitative real-time PCR. This study provides new insights into the early immune response of a host that is resistant to C. irritans.