Journal
FEBS JOURNAL
Volume 286, Issue 14, Pages 2711-2725Publisher
WILEY
DOI: 10.1111/febs.14840
Keywords
DNA polymerases; PCNA; Rev1; translesion DNA synthesis; ubiquitination
Categories
Funding
- Chinese Ministry of Science and Technology National 973 Project [2013CB911003]
- Capital Normal University
- Capital Normal University Entrance Scholarship
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Rev1, a Y-family DNA polymerase, is involved in the tolerance of DNA damage by translesion DNA synthesis (TLS). Previous studies have shown that the C-terminal domain (CTD) and ubiquitin (Ub)-binding (UBM) domains of Rev1 play important roles in UV-damage tolerance, but how these domains contribute to the process remains unclear. In this study, we created Ub mutations in a proliferating cell nuclear antigen (PCNA)-Ub fusion that differentially affect its interaction with Rev1 and Pol eta and found that UV-damage tolerance depends on its interaction with Rev1 but not Pol eta. We also created Rev1-UBM mutations altering its interaction with a PCNA-Ub fusion and Rev1-CTD mutations affecting its interaction with Pol eta and the Rev7 subunit of Pol zeta. We thus demonstrated that elevated expression of Rev1 alone is sufficient to confer enhanced UV-damage tolerance and that this tolerance depends on its physical interaction with monoubiquitinated PCNA and Pol zeta but is independent of Pol eta. Collectively, these studies reveal central roles played by Rev1 in coordinating UV-damage response pathway choice in mammalian cells.
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