Promoting bacteria-anode interfacial electron transfer by palladium nano-complex in double chamber microbial fuel cell

The slow electron transfer between microbial outer membrane and electrode surface is considered one of the limitations of Microbial Fuel Cell (MFC) performance. The aim of the present work is to assess the role of palladium alpha-lipoic acid nanocomplex compound (PLAC) in promoting bacteria-anode interfacial electron transfer, by studying the dielectric properties of Shewanella oneidensis WW-1 cell membrane and its contribution to biofilm formation on the anode. The results showed that adding PLAC increased bacterial cell membrane permeability and outer cell surface charge. Exopolysaccharides (EPS) and surface-bound proteins increased 2.27 and 1.14 fold, respectively upon adding 0.25% v/v PLAC. Dynamic Light Scattering (DLS) showed uniform distribution of Shewanella-PLAC biocomposite size while Zeta potential and Fourier Transform Infrared (FTIR) Spectroscopy results suggest that PLAC diffused inside the cells. Transmission Electron Microscope (TEM) images reveal Exopolysaccharide (EPS) mat around the cells when PLAC was added to the cells, also confirmed by the FTIR spectrum. Scanning Electron Microscope and Atomic Force Microscope (AFM) confirmed the thickness of biofilm in the presence of PLAC. The average voltage reached 492 mV (external resistance 1 K omega) over 35 days using 0.25% v/v PLAC as compared to a few hours in MFCs lacking PLAC. The results suggest that the addition of PLAC assisted in interfacial direct electron transfer through enhancing biofilm formation, moreover, its hydrophilic/lipophilic nature facilitated the electron shuttling process from within the bacterial cell to the electrode surface suggesting the involvement of mediated electron transfer as well. [GRAPHICS] .

Automated summary

The addition of palladium alpha-lipoic acid nanocomplex compound (PLAC) was found to increase bacterial cell membrane permeability and outer cell surface charge, facilitating biofilm formation and interfacial electron transfer.