Journal
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Volume 26, Issue 19, Pages 19560-19574Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11356-019-05357-x
Keywords
Nano-toxicity; Bristle defect; Wing spot assay; Climbing assay; Light; dark assay; Comet assay
Categories
Funding
- MHRD
- DST-Inspire
- DBT [BT/PR21857/NNT/28/1238/2017]
Ask authors/readers for more resources
The current study checks the effect of various concentrations of dietary graphene oxide (GO) nano-sheets on the development of Drosophila melanogaster. GO was synthesized and characterized by XRD, FTIR, FESEM, and TEM analytical techniques. Various concentrations of GO were mixed with the fly food and flies were transferred to the vial. Various behavioral and morphological as well as genetic defects were checked on the different developmental stages of the offspring. In the larval stage of development, the crawling speed and trailing path change significantly than the control. GO induces the generation of oxygen radicals within the larval hemolymph as evidenced by nitroblue tetrazolium assay. GO induces DNA damage within the gut cell, which was detected by Hoechst staining and within hemolymph by comet assay. Adult flies hatched after GO treatment show defective phototaxis and geotaxis behavior. Besides behavior, phenotypic defects were observed in the wing, eye, thorax bristles, and mouth parts. At 300 mg/L concentration, wing spots were observed. Altogether, the current study finds oral administration of GO which acts as a mutagen and causes various behavioral and developmental defects in the offspring. Here for the first time, we are reporting GO, which acts as a teratogen in Drosophila, besides its extensive medical applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available