Journal
ENVIRONMENTAL SCIENCE & TECHNOLOGY
Volume 53, Issue 12, Pages 6660-6668Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.9b01345
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Funding
- Australian Research Council (ARC) [FL170100086, DP170104038]
- U.S. Department of Energy's Office of Biological Environmental Research [DE-SC0010574]
- University of Queensland International Scholarship (UQI)
- UQ Research Training Scholarship
- University of Queensland International Scholarship
- China Scholarship Council Scholarship
- Advanced Queensland Research Fellowship
- U.S. Department of Energy (DOE) [DE-SC0010574] Funding Source: U.S. Department of Energy (DOE)
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Biochar was recently identified as an effective soil amendment for CH4 capture. Corresponding mechanisms are currently recognized to be from physical properties of biochar, providing a favorable growth environment for aerobic methanotrophs which perform aerobic methane (CH4) oxidation. However, our study shows that the chemical reactivity of biochar can also stimulate anaerobic oxidation of CH4 (AOM) by anaerobic methanotrophic archaea (ANME) of ANME-2d, which proposes another plausible mechanism for CH4 mitigation by biochar amendment in anaerobic environments. It was found that, by adding biochar as the sole electron acceptor in an anaerobic environment, CH4 was biologically oxidized, with CO2 production of 106.3 +/- 5.1 mu mol g(-1) biochar. In contrast, limited CO2 production was observed with chemically reduced biochar amendment. This biological nature of the process was confirmed by mcr gene transcript abundance as well as sustained dominance of ANME-2d in the microbial community during microbial incubations with active biochar amendment. Combined FTIR and XPS analyses demonstrated that the redox activity of biochar is related to its oxygen-based functional groups. On the basis of microbial community evolution as well as intermediate production during incubation, different pathways in terms of direct or indirect interactions between ANME-2d and biochar were proposed for biochar-mediated AOM.
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