Journal
ENVIRONMENTAL POLLUTION
Volume 248, Issue -, Pages 599-608Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2019.02.080
Keywords
Docking; Active site; Textile dyes; Aeromonas hydrophila; Lysinibacillus sphaericus; Biodegradation
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Funding
- Kalasalingam University, Krishnankoil
- National College, India (Autonomous), Tiruchirappalli, India
- Science and Engineering Research Board (SERB), New Delhi [SR/FT/LS-121/2011]
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Growth of textile industries led to production of enormous dye varieties. These textile dyes are largely used, chemically stable and easy to synthesize. But they are recalcitrant and persist as less biodegradable pollutants when discharged into waterbodies. Potential use of enzyme-linked bioremediation of textile dyes will control their toxicity in waterbodies. Bioinformatics and Molecular docking tool provides an insight into remediation mechanism by predicting susceptibility of dye degradation using oxidoreductive enzymes. In this study, six dyes, Reactive Red F3B, Remazol Red RGB, Joyfix Red RB, Joyfix Yellow MR, Remazol Blue RGB and Turquoise CL-5B of azo, anthraquinone and phthalocyanine molecular class were identified as potential targets for degradation by laccase and azoreductase of Aeromonas hydrophila in addition to Lysinibacillus sphaericus through in silico docking tool BioSolveIT-FlexX. Azoreductase breaks azo bonds by ping-pong mechanism whereas laccase decolorizes dyes by free radical mechanism which is not specific in nature. Results were analyzed based on parameters like stability, catalytic action and selectivity for enzyme-dye interactions. Amino acids of enzymes interacted with several dyes substantiating variations in active site for enzyme-ligand binding affinity. This suggests the role of enzymes in decolorizing an extensive variety of textile dyes, thereby, aiding in understanding the enzyme mechanisms in Bioremediation. (C) 2019 Elsevier Ltd. All rights reserved.
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