Journal
ENVIRONMENTAL MICROBIOLOGY
Volume 21, Issue 8, Pages 2933-2947Publisher
WILEY
DOI: 10.1111/1462-2920.14686
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Funding
- National Science Foundation of China [41831287, 31670130, 31600110] Funding Source: Medline
- Science and Technology Committee of Tianjin [17JCQNJC09200] Funding Source: Medline
- Science and Technology Program of Sichuan Province [2018JZ0069] Funding Source: Medline
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The biofilm formation by Pseudomonas aeruginosa highly increases the bacterial resistance to antimicrobial agents and host immune clearance. The biofilm formation is positively regulated by two small RNAs, RsmY and RsmZ. Previously, we reported that mutation in the polynucleotide phosphorylase (PNPase) coding gene pnp increases the levels of RsmY/Z. However, in this study, we found that the biofilm formation is decreased in the pnp mutant, which is due to a defect in rhamnolipids production. The rhamnolipids production is regulated by the RhlI-RhlR quorum sensing system. We found that PNPase influences the translation of RhlI through its 5 '-untranslated region (UTR) and identified that the sRNA P27 is responsible for the translational repression. In vitro translation experiments demonstrated that P27 directly represses the translation of the rhlI mRNA through its 5 ' UTR in an Hfq-dependent manner. Point mutations in the rhlI 5 ' UTR or P27, which abolish the pairing between the two RNAs restore the rhlI expression and rhamnolipids production as well as the biofilm formation in the pnp mutant. Overall, our results reveal a novel layer of regulation of the Rhl quorum sensing system by the sRNA P27.
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