Journal
ELECTROPHORESIS
Volume 40, Issue 16-17, Pages 2075-2083Publisher
WILEY
DOI: 10.1002/elps.201900013
Keywords
Capillary electrophoresis; Enzyme inhibitor; Immobilization; Screening
Funding
- National Natural Science Foundation of China [81711540311, 81673325, 21705156] Funding Source: Medline
- International Science and Technology Cooperation Project of Gansu Key R&D Program [18YF1WA127, CAS Pioneer Hundred Talents Program] Funding Source: Medline
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Capillary electrophoresis (CE) has attracted lots of attention due to its simplicity, low sample consumption, low solvent volume, high resolution, and high speed. Based on these advantages, it has been widely used in enzyme inhibitor screening. There are two main operation modes on enzyme inhibitor screening: off-line (precapillary enzyme assays) in which process CE was used as an analytical tool; online (in-capillary enzyme assays) which combined the sample injection, mix, reaction, separation, and detection within a single run. Additionally, diverse of new materials were introduced to immobilize enzyme, which has been coupled with CE for the study of enzyme activity and its inhibitor screening. This review gives an overview of the developments and applications for the CE-based enzyme inhibitor screening.
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