Journal
DEVELOPMENTAL CELL
Volume 49, Issue 2, Pages 267-+Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2019.03.014
Keywords
-
Categories
Funding
- JSPS KAKENHI [16H06166, 17K19362]
- TAKEDA Science Foundation
- Science Research on Innovative Areas Molecular Engines'' [18H05427]
- JSPS Postdoctoral Fellowship
- Grants-in-Aid for Scientific Research [17K19362, 16H06166] Funding Source: KAKEN
Ask authors/readers for more resources
The microtubule-based spindle is subjected to various mechanical forces during cell division. How the structure generates and responds to forces while maintaining overall integrity is unknown because we have a poor understanding of the relationship between filament architecture and mechanics. Here, to fill this gap, wecombine microneedle-based quantitative micromanipulation with high-resolution imaging, simultaneously analyzing forces and local filament motility in the Xenopus meiotic spindle. We find that microtubules exhibit a compliant, fluid-like mechanical response at the middle of the spindle half, being distinct from those near the pole and the equator. A force altering spindle length induces filament sliding at this compliant array, where parallel microtubules predominate, without influencing equatorial antiparallel filament dynamics. Molecular perturbations suggest that kinesin-5 and dynein contribute to the spindle's local mechanical difference. Together, our data establish a link between spindle architecture and mechanics and uncover the mechanical design of this essential cytoskeletal assembly.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available