Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 94, Issue -, Pages 66-72Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2019.01.014
Keywords
AMPs; BmBr-C Z2; Bombyx mori; Insect immunity; 20E
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Funding
- National Natural Science Foundation of China [31672496, 31802142, 81872071, 81672502]
- China Postdoctoral Science Foundation [2017M620408]
- Natural Science Foundation of Chongqing [cstc2016jcyjA0425]
- Chongqing University Innovation Team Building Program [CXTDX201601010]
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Broad-Complex Z2 (Br-C Z2) is an ecdysone inducible transcription factor that regulates physiological, innate immune and developmental events in insects. Here, we identified an orthologue of Br-C Z2 from silkworm, Bombyx mori (BmBr-C Z2) to study its involvement in immune responses. The quantitative real-time PCR analysis revealed that BmBr-C Z2 was expressed ubiquitously in all tested tissues under normal physiological conditions. Further, developmental profile displayed that BmBr-C Z2 expression was detectable in different developmental stages, however the genes expression was highest in the molting and pre-pupal stages. Administration of 20hydroxyecdysone (20E) enhanced the expression levels of BmBr-C Z2 in hemocytes. The challenge with pathogens and pathogen associated molecular patterns (PAMPs) also upregulated the mRNA levels of BmBr-C Z2 in hemocytes when compared with the control. By contrast, the ectopic expression of BmBr-C Z2 remarkably increased the production of antimicrobial peptides, while the knock-down of this gene by double stranded RNA decreased their production. Dual-luciferase assay exhibited that BmBr-C Z2 induced the expression of lysozyme by directly binding to its promoter region. The treatment of Escherichia coli following the knock-down of BrnBr-C Z2 strongly reduced the survival rate of silkworm larvae. These results suggest that BmBr-C Z2 plays an important biological role in the innate immune responses of silkworm by regulating immune-related genes.
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