Journal
DEVELOPMENT
Volume 146, Issue 7, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/dev.170498
Keywords
Cdx; Mouse; Mesoderm; Cell fate; Transcription; Chromatin
Categories
Funding
- Canadian Institutes of Health Research [220871]
- Ontario Graduate Scholarship - Government of Ontario
- Ontario Graduate Scholarship - University of Ottawa
Ask authors/readers for more resources
Murine cardiac and hematopoletic progenitors are derived from Mesp1(+) mesoderm. Cdx function impacts both yolk sac hematopoiesis and cardiogenesis in zebrafish, suggesting that Cdx family members regulate early mesoderm cell fate decisions. We found that Cdx2 occupies a number of transcription factor loci during embryogenesis, including key regulators of both cardiac and blood development, and that Cdx function is required for normal expression of the cardiogenic transcription factors Nkx2-5 and Tbx5. Furthermore, Cdx and Brg1, an ATPase subunit of the SWI/SNF chromatin remodeling complex, cooccupy a number of loci, suggesting that Cdx family members regulate target gene expression through alterations in chromatin architecture. Consistent with this, we demonstrate loss of Brg 1 occupancy and altered chromatin structure at several cardiogenic genes in Cdx-null mutants. Finally, we provide evidence for an onset of Cdx2 expression at E6.5 coinciding with egression of cardiac progenitors from the primitive streak. Together, these findings suggest that Cdx functions in multi-potential mesoderm to direct early cell fate decisions through transcriptional regulation of several novel target genes, and provide further insight into a potential epigenetic mechanism by which Cdx influences target gene expression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available