Journal
CROP PROTECTION
Volume 118, Issue -, Pages 36-43Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.cropro.2018.12.009
Keywords
Pythium root rot; rDNA-ITS regions; Cytochrome oxidase gene; Aggressiveness; Soybeans
Categories
Funding
- Government of Canada through the Agri-Flex Program
- Agriculture and Agri-Food Canada (Pulse Science Cluster)
- Saskatchewan Pulse Growers
- Manitoba Pulse and Soybean Growers
- Young Talents Training Program of Shandong Academy of Agricultural Sciences
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Soybean (Glycine max (L.) Merr.) has been a commercial oilseed crop in Canada since the 1920s. The production of soybean rapidly increased from 2004 to 2016 in Alberta and Manitoba. Root rot disease caused by the oomycete genus Pythium was observed from 105 fields in 2015 and 2016 in southern Alberta and Manitoba. In the present study, a total of 97 isolates of Pythium were recovered from the root tissues of soybean seedling collected from those fields. Identification of the Pythium species was achieved by examining their morphological features and sequences analysis of internal transcribed spacers (ITS) and cytochrome oxidase subunit 2 gene (Cox II). Nine species of Pythium were identified, with Pythiwn violae as the predominant species (27 out of 97, 27.8%), followed by P. intermedium (21), P. irregulare (16), P. sylvaticum (12), P. recalcitrans (8), P. ultimum (8), P. heterothallicum (3), P. conidiophorum (1) and P. macrosporum (1). This is the first report of P. conidiophorum, P. recalcitrans and P. violae on soybean in Canada. All the Pythium isolates except three isolates (S2d of P. violae, S29a and S29b of P. heterothallicum) were highly virulent on soybean, causing root rot disease symptoms.
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