Journal
COMPARATIVE CYTOGENETICS
Volume 13, Issue 2, Pages 121-132Publisher
PENSOFT PUBLISHERS
DOI: 10.3897/CompCytogen.v13i2.34116
Keywords
meiosis; microdissection; sex chromosomes; lampbrush chromosomes; heterochromatin; lizard
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Funding
- Federal Agency of Scientific Organizations via the Institute of Cytology and Genetics [0324-2019-0042]
- Russian Foundation for Basic Research [18-34-00182]
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Reptiles are good objects for studying the evolution of sex determination, since they have different sex determination systems in different lineages. Lacertid lizards have been long-known for possessing ZZ/ZW type sex chromosomes. However, due to morphological uniformity of lacertid chromosomes, the Z chromosome has been only putatively cytologically identified. We used lampbrush chromosome (LBC) analysis and FISH with a W-specific probe in Eremias velox (Pallas, 1771) to unequivocally identify the ZW bivalent and investigate its meiotic behavior. The heterochromatic W chromosome is decondensed at the lampbrush stage, indicating active transcription, contrast with the highly condensed condition of the lampbrush W chromosomes in bunts. We identified the Z chromosome by its chiasmatic association with the W chromosome as chromosome XIII of the 19 chromosomes in the LBC karyotype. Our findings agree with previous genetic and genomic studies, which suggested that the lacertid Z chromosome should be one of the smaller macrochromosomes.
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