Journal
CLINICAL PHARMACOLOGY & THERAPEUTICS
Volume 106, Issue 4, Pages 831-840Publisher
WILEY
DOI: 10.1002/cpt.1472
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- CIHR [299309] Funding Source: Medline
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The relevance of endogenous 4 beta-hydroxycholesterol (4 beta-OHC) plasma concentrations or of the 4 beta-OHC/total cholesterol concentration ratio (4 beta-OHC ratio) as surrogate markers of cytochrome P450 3A (CYP3A) activity was evaluated in individuals with (n = 38) or without (n = 35) type 2 diabetes (T2D). Midazolam was used as a comparator to validate exploratory measures of phenotypic CYP3A activity. Metabolic ratios of orally administered midazolam in nondiabetic and diabetic populations correlated significantly with 4 beta-OHC (r(s) = 0.64 and 0.48; P <= 0.003) and 4 beta-OHC ratio (r(s) = 0.69 and 0.46; P <= 0.003), respectively. Activity of CYP3A was lower in the T2D population compared with nondiabetic subjects; this decrease was reflected in 4 beta-OHC concentrations (24.33 vs. 12.58 ng/mL; P < 0.0001) and 4 beta-OHC ratio (0.13 vs. 0.09 (x 10(4)); P < 0.0002). These results suggest that 4 beta-OHC should be considered as a valid, convenient, and easy to use endogenous biomarker of CYP3A activity in patients.
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