Journal
ANNALS OF DERMATOLOGY
Volume 28, Issue 5, Pages 579-585Publisher
KOREAN DERMATOLOGICAL ASSOC
DOI: 10.5021/ad.2016.28.5.579
Keywords
CpG; Interleukin-17A; Keratinocytes; NF-kappa B; Psoriasis
Categories
Funding
- National Research Foundation of Korea [NRF-2012R1A1A2008920]
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Background: Interleukin-17A (IL-17A) is mainly secreted from Th17 cells that are activated by various stimuli including CpG oligodeoxynucleotide, a Toll-like receptor 9 (TLR9) ligand. Recently, it has been demonstrated that keratinocytes play an important role in the pathogenesis of psoriasis. Objective: To investigate the potential role of keratinocytes, we examined whether TLR9 ligand CpG induces IL-1.7A expression in keratinocytes. Methods: We used HaCaT keratinocytes as a model system, and determined CpG-induced IL-17A using enzyme-linked immunosorbent assay and Western blot. Results: When HaCaT keratinocytes were treated with CpG, the expression of several cytokines including IL-17A, tumor necrosis factor- alpha and CCL20 was markedly increased. Treatment with nuclear factor (NF)- kappa B inhibitor significantly blocked the CpG-induced IL-17A production, indicating that CpG induced IL-17A expression through the NF- kappa B signaling pathway. In addition, IL-17A secreted from keratinocytes stimulated the CD4(+) T cells, resulting in strong induction of IL-22 production. Conclusion: Since IL-22 is an important mediator for psoriatic inflammation, our data suggest that keratinocytes can participate in the pathogenesis of psoriasis via the TLR9-dependent IL-17A production.
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