Journal
CIENCIA RURAL
Volume 49, Issue 4, Pages -Publisher
UNIV FEDERAL SANTA MARIA
DOI: 10.1590/0103-8478cr20180639
Keywords
Antimicrobial; Phytochemical Screening; Phenolic compounds
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Funding
- Brazilian federal government Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior(CAPES)
- Brazilian federal government Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE)
- Brazilian federal government Universidade Federal Rural de Pernambuco(UFRPE)
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This study aimed to perform phytochemical analysis and to test the antimicrobial activity of the crude hydroalcoholic extract obtained from the leaves of Sphagneticola trilobata. Classes of secondary metabolites present in the extract were identified through phytochemical screening using analytical thin-layer chromatography. Antimicrobial activity was evaluated by testing cultures of Staphylococcus aureus, S. epidermidis, Staphylococcus spp., Escherichia coli, Serratia marcescens, Enterococcus faecalis, Pseudomonas aeruginosa, Salmonella Typhimurium, and Klebsiella pneumoniae isolated from human skin and those of Staphylococcus spp. isolated from dog skin using the broth microdilution method. In the phytochemical screening, classes of anthracenic derivatives and mono-, sesqui-, and diterpenes were identified. Colorimetric analysis showed total phenol and total flavonoid contents of 21.7 +/- 0.009 mg of gallic acid equivalents per gram of sample and 0.23 +/- 0.005 mg of catechin equivalents per gram of sample, respectively. Microbiological analysis revealed that the hydroalcoholic extract of S. trilobata exhibited antimicrobial activity against cultures of Staphylococcus spp., E. coli, S. marcescens, and E. faecalis isolated from human skin and those of Staphylococcus spp. isolated from dog skin. Thus, crude hydroalcoholic extract of leaves of S. trilobata contained flavonoids and terpenoids as secondary metabolites, which contributed to its antimicrobial activity against skin bacteria isolated from different sources.
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