Journal
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
Volume 35, Issue 3, Pages 382-389Publisher
HIGHER EDUCATION PRESS
DOI: 10.1007/s40242-019-8322-3
Keywords
Ochratoxin A; Fe3O4; Magnetic separation; Fluorescence method; Aptamer
Categories
Funding
- National Natural Science Foundation of China [21305032]
- China Postdoctoral Science Foundation [2014M551522]
- Postdoctoral Science Foundation of Jiangsu Province, China [1402073B]
- Henan Key Laboratory of Biomolecular Recognition and Sensing(Shangqiu Normal University), China [HKLBRSK1803]
- Hong Kong Scholar Program, China [XJ2017008]
Ask authors/readers for more resources
A double magnetic separation-assisted fluorescence method was developed to rapidly detect ochratoxin A(OTA). The OTA aptamer functionalized magnetic nanomaterial(Fe3O4-Aptamer) and complementary DNA conjugated nitrogen-doped graphene quantum dots(NGQDs-cDNA) were used in this assay. Aptamer could hybridize with cDNA, which induced the NGQDs-cDNA to bind onto Fe3O4-Aptamer, and resulted in the fluorescence quenching of NGQDs. After the addition of OTA, the NGQDs-cDNA could release into the solution, and resulted in the recovery of fluorescence signal of NGQDs consequently. By utilizing the magnetic separation, the unbonded NGQDs-cDNA and residual Fe3O4-Aptamer were removed, which significantly increased the fluorescence signal intensity. OTA could be detected in the linear range of 10 nmol/L to 2000 nmol/L, with a limit of detection as 0.66 nmol/L. The advantages of this method include simple operation, good selectivity and high sensitivity, and this method can be used for the rapid detection of ochratoxin A in wheat and corn.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available