Journal
CARBOHYDRATE POLYMERS
Volume 220, Issue -, Pages 236-246Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2019.05.011
Keywords
Capture; Design of experiments; Gellan microspheres; Protein; Sphingomonas paucimobilis; Water-in-oil emulsion
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Funding
- FEDER funds through the POCI - COMPETE 2020 -Operational Programme Competitiveness and Internationalization in Axis I - Strengthening research, technological development and innovation [POCI-01-0145-FEDER-007491]
- FCT - Foundation for Science and Technology [UID/Multi/00709/2013]
- Applied Molecular Biosciences Unit-UCIBIO - FCT/MCTES [UID/Multi/04378/2019]
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In the present work, the biosynthesis of the gellan gum exopolysaccharide from Sphingomonas paucimobilis fermentation was improved to 24 g/L, by increasing the oxygen content (1:5). FTIR and NMR data indicated the following procedures to recover gellan from culture medium:filtration; washing with acetone and ether (200 mL each one); permeate dissolution with distilled water; precipitation with acetonitrile (1:3). Gellan microsphere formulation by water-in-oil emulsion was optimized through central composite design to minimize the diameter (approximate to 300 mu m), and reinforced with different counter-ions. The optimal point was reached with 1.27% gellan concentration, 750 rpm and 92.54 degrees C of oil temperature. Microspheres with nickel as counter-ion revealed the most satisfactory results from semi-optical microscopy, SEM and EDX analysis. The manipulation of ionic strength or pH on the binding and elution steps allowed capturing different model proteins, exploring electrostatic or affinity interactions with these microspheres.
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