Journal
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
Volume 208, Issue -, Pages 129-134Publisher
ELSEVIER GMBH
DOI: 10.1016/j.aanat.2016.05.006
Keywords
Surfactant proteins; SFTA3; Escherichia coli; TA-Cloning
Categories
Funding
- German Research Foundation (DFG) [BR 1329/12-1, BR 3681/2-1, PA738/9-2]
- Ernst and Berta Grimmke Stiftung [1/15]
- Sicca Forschungsforderung of the Professional Association of German Ophthalmologists
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Surfactant proteins are broadly understood to be an important structural and functional part of the lung surfactant system. These proteins influence the intra-alveolar surface tension and contribute to innate immunity of the lung. Beside the four already known surfactant proteins SP-A, SP-B, SP-C and SP-D, two novel SPs (SP-G/SFTA2 and SP-H/SFTA3) have recently been described. These show no sequential or structural similarity with the already known SPs. However, bioinformatic prediction tools suggest physicochemical properties, which have already been described for other surfactant proteins. Although it is known that SFTA2 and SFTA3 are expressed in different human tissues, their distinct functional properties remain unclear. Here, we describe the establishment of a stable expression system for recombinant SFTA3 protein synthesis in Escherichia coli. This gives rise to future experiments with the overall aim to further examine and characterize this novel protein in more detail. (C) 2016 Elsevier GmbH. All rights reserved.
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