Journal
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
Volume 52, Issue 5, Pages -Publisher
ASSOC BRAS DIVULG CIENTIFICA
DOI: 10.1590/1414-431X20197992
Keywords
Artesunate; Ovarian cancer; CD4(+) T cells; Th1 cells; miR-142; Sirt1
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The aim of this study was to evaluate the influence of artesunate on Th1 differentiation and its anti-tumor effect on ovarian cancer. A Murine ovarian cancer model was established by ID8 cells transplantation. The expression of miR-142 and Sirt1 proteins in peripheral CD4(+) T cells were quantified with qRT-PCR and western blot, respectively. Peripheral CD4(+) T cells were induced for Th1 differentiation. The percentages of apoptosis of Th1/CD4(+) T cells and ovarian cancer cells were analyzed by flow cytometry. The IFN-gamma level was examined through enzyme-linked immunosorbent assay. Artesunate promoted miR-142 expression in peripheral CD4(+) T cells and Th1 differentiation from CD4(+) T cells. Artesunate promoted cell apoptosis of ovarian cancer cells by inducing Th1 differentiation. By up-regulating miR-142, artesunate suppressed Sirt1 level and promoted Th1 differentiation. Artesunate enhanced the pro-apoptotic effects of Th1 cells on ovarian cancer via the miR-142/Sirt1 pathway. Artesunate promoted Th1 differentiation from CD4(+) Tcells by down-regulating Sirt1 through miR-142, thereby enhancing cell apoptosis in ovarian cancer.
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