Journal
BMC GENOMICS
Volume 20, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12864-019-5769-z
Keywords
Barbarea vulgaris; Restriction site-associated DNA sequencing (RAD-Seq); Genetic linkage map; QTL; Leaf traits; Glucosinolates
Funding
- National Key Technology R & D Program of the Ministry of Science and Technology of China [2013BAD01B04-2]
- Agriculture Research System of the Ministry of Agriculture and Rural Affairs of China [CARS-24-A-01]
- Henan Provincial Science and Technology Open Cooperation Project of the Department of Science and Technology of Henan Province [182106000052]
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BackgroundBarbarea vulgaris is a wild cruciferous plant and include two distinct types: the G- and P-types named after their glabrous and pubescent leaves, respectively. The types differ significantly in resistance to a range of insects and diseases as well as glucosinolates and other chemical defenses. A high-density linkage map was needed for further progress to be made in the molecular research of this plant.ResultsWe performed restriction site-associated DNA sequencing (RAD-Seq) on an F-2 population generated from G- and P-type B. vulgaris. A total of 1545 SNP markers were mapped and ordered in eight linkage groups, which represents the highest density linkage map to date for the crucifer tribe Cardamineae. A total of 722 previously published genome contigs (50.2Mb, 30% of the total length) can be anchored to this high density genetic map, an improvement compared to a previously published map (431 anchored contigs, 38.7Mb, 23% of the assembly genome). Most of these (572 contigs, 31.2Mb) were newly anchored to the map, representing a significant improvement. On the basis of the present high-density genetic map, 37 QTL were detected for eleven traits, each QTL explaining 2.9-71.3% of the phenotype variation. QTL of glucosinolates, leaf size and color traits were in most cases overlapping, possibly implying a functional connection.ConclusionsThis high-density linkage map and the QTL obtained in this study will be useful for further understanding of the genetic of the B. vulgaris and molecular basis of these traits, many of which are shared in the related crop watercress.
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