Journal
BIOTECHNOLOGY ADVANCES
Volume 37, Issue 5, Pages 730-745Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.biotechadv.2019.04.002
Keywords
Industrial biotechnology; Synthetic biology; Chromosomal integration; CRISPR-Cas; Site-specific recombination; Homology-mediated end Joining
Categories
Funding
- National Natural Science Foundation of China [31630003]
- National Postdoctoral Program for Innovative Talents [BX201700265]
- China Postdoctoral Science Foundation [2017M621545]
- Science and Technology Commission of Shanghai Municipality [18ZR1446700]
- National Mega -project for Innovative Drugs [2018ZX09711001-006-012]
- Derivative Bank of Chinese Biological Resources, CAS [ZSYS-016]
Ask authors/readers for more resources
Industrial biotechnology is reliant on native pathway engineering or foreign pathway introduction for efficient biosynthesis of target products. Chromosomal integration, with intrinsic genetic stability, is an indispensable step for reliable expression of homologous or heterologous genes and pathways in large-scale and long-term fermentation. With advances in synthetic biology and CRISPR-based genome editing approaches, a wide variety of novel enabling technologies have been developed for single-step, markerless, multi-locus genomic integration of large biochemical pathways, which significantly facilitate microbial overproduction of chemicals, pharmaceuticals and other value-added biomolecules. Notably, the newly discovered homology-mediated end joining strategy could be widely applicable for high-efficiency genomic integration in a number of homologous recombination-deficient microbes. In this review, we explore the fundamental principles and characteristics of genomic integration, and highlight the development and applications of targeted integration approaches in the three representative industrial microbial systems, including Escherichia colt actinomycetes and yeasts.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available