Journal
BIOSENSORS & BIOELECTRONICS
Volume 133, Issue -, Pages 192-198Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.03.035
Keywords
Copper doped tungsten trioxide; Electrochemiluminescence; Luminol; Resonance energy transfer; Amyloid beta-protein
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Funding
- Natural Science Foundation of Shandong Province, China [ZR2017BB030]
- National Key Scientific Instrument and Equipment Development Project of China [21627809]
- National Natural Science Foundation of China [21575050, 21777056, 21505051]
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A highly efficient quench-type electrochemiluminescence (ECL) immunosensor was proposed for the trace detection of amyloid beta-protein (A beta(1-42)). In this work, tin disulfide nanoflowers (SnS2-NFs) with large specific surface area, favorable catalytic property and chemical stability were prepared and used as substrate material. Taking advantage of the excellent catalytic ability and biocompatibility, palladium nanoparticles (Pd NPs) were in situ reduced on SnS2 NFs to obtain SnS2-Pd. Moreover it could combine with large amounts of luminol and achieve a strong ECL signal output. In addition, copper doped mesoporous tungsten trioxide (Cu:WO3) nanoparticles were selected to quench ECL emission of luminol@SnS2-Pd via resonance energy transfer, where luminol@SnS2-Pd was the donor and Cu:WO3 was the acceptor. On this basis, a quench-type ECL immunosensor was constructed for detection of A beta(1-42). Under optimum conditions, the fabricated ECL immunosensor showed sensitive response to A beta(1-42) concentration from 0.1 pg/mL to 50 ng/mL with a low detection limit of 5.4 fg/mL (S/N = 3). It is expected to be a promising analytical tool for the sensitive detection of A beta(1-42) and other biomarkers with high specificity, good reproducibility and long-term stability.
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