Journal
BIOSENSORS & BIOELECTRONICS
Volume 132, Issue -, Pages 8-16Publisher
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2019.02.040
Keywords
Covalent organic framework; Label-free aptasensor; Detection of enrofloxacin; Detection of ampicillin; Electrochemical impedance spectroscopy
Categories
Funding
- Programs for the National Natural Science Foundation of China (NSFC) [U1604127, 21601161]
- Innovative Technology Team of Henan Province [CXTD2014042]
- Scientific and Technological Project of Henan Province [192102310261, 192102310460]
- Young Backbone Teacher Training Program in Universities of Henan Province [2018GGJS089]
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We designed and synthesized a novel covalent organic framework (COF) by condensation polymerization of 1,3,6,8-tetrakis(4-formylphenyl)pyrene and melamine through imine bonds (represented by Py-M-COF). The basic characterizations revealed that the Py-M-COF not only exhibited an extended pi-conjugation framework, a large specific surface area (495.5 m(2) g(-1)), big pore cavities, and nanosheet-like structure but also possessed rich functional groups, such as (CC)-C-center dot, (CN)-N-center dot, (CO)-O-center dot and NH2. These features endowed the Py-M-COF with high charge carrier mobility, further improving the strong immobilization of DNA aptamer strands via pi-pi stacking interaction and electrostatic interaction. As such, the Py-M-COF-based electrochemical aptasensors are ultrasensitive in detecting different antibiotics, including enrofloxacin (ENR) and ampicillin (AMP), yielding extremely low detection limits of 6.07 and 0.04 fg mL(-1) (S/N = 3) toward ENR and AMP, respectively, along with other excellent sensing performances. This biosensing platform based on Py-M-COF has potential applications for the sensitive detection of antibiotics or other analytes by replacing the corresponding aptamers.
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