Journal
BIORESOURCE TECHNOLOGY
Volume 278, Issue -, Pages 17-25Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2019.01.041
Keywords
Microalgae; Semi-continuous; Fed-batch; Lutein; Mixotrophic growth; Photobioreactor; Outdoor cultivation
Funding
- Taiwan's Ministry of Science and Technology [107-3113-E-006-009, 107-2221-E-006-112-MY3, 107-2621-M-006-003, 107-2218-E-006-016]
- DDS Institute of Advanced Education, Taiwan
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A lutein-enriched mutant, Chlorella sorokiniana MB-1-M12 was grown mixotrophically for lutein production. The lutein production efficiency of the strain was enhanced via optimizing the operating strategies. The results show that using semi-continuous cultivation with a medium replacement ratio of 75% resulted in a higher lutein productivity and lutein concentration of 6.24 mg/L/d and 50.6 mg/L, respectively, which were markedly higher than those obtained from batch and fed-batch cultivation. Cultivation under simulated outdoor cultivation conditions (i.e., temperature of 35 degrees C/25 degrees C for a 12 h/12 h light/dark cycle) could achieve the highest lutein productivity and lutein concentration of 3.34 mg/L/d and 30.8 mg/L, respectively. Lutein production via outdoor cultivation of MB-1-M12 strain with a 60-L tubular photobioreactor was performed using semi-continuous operation. With a medium replacement ratio of 75%, a good lutein productivity (4.46 mg/L/d) and concentration (27.4 mg/L) was obtained, indicating the feasibility of producing lutein under outdoor cultivation of the microalgal strain.
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