Journal
BIOMATERIALS
Volume 199, Issue -, Pages 22-31Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2019.01.042
Keywords
Upconversion materials; Near-infrared (NIR) light; Optogenetics; Autophagy; Protein-protein interaction (PPI)
Funding
- National Key Research and Development Program of China [2017YFA0205104]
- National Natural Science Foundation of China [81771970, 51573128]
- Tianjin Development Program for Innovation and Entrepreneurship
- Young Elite Scientists Sponsorship Program by Tianjin
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In vivo noninvasively manipulating biological functions by the mediation of biosafe near infrared (NIR) light is becoming increasingly popular. For these applications, upconversion rare-earth nanomaterial holds great promise as a novel photonic element, and has been widely adopted in optogenetics. In this article, an upconversion optogenetic nanosystem that was promised to achieve autophagy up-regulation with spatiotemporal precision was designed. The implantable, wireless, recyclable, less-invasive and biocompatible system worked via two separated parts: blue light-receptor optogenetics-autophagy upregulation plasmids, for protein import; upconversion rods-encapsulated flexible capsule (UCRs-capsule), for converting tissue-penetrative NIR light into local visible blue light. Results validated that this system could achieve up-regulation of autophagy in vitro (in both HeLa and 293T cell lines) and remotely penetrate tissue (similar to 3.5 mm) in vivo. Since autophagy serves at a central position in intracellular signalling pathways, which is correlative with diverse pathologies, we expect that this method could establish an upconversion material-based autophagy up-regulation strategy for fundamental and clinical applications.
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