Journal
BIOMACROMOLECULES
Volume 20, Issue 6, Pages 2185-2197Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.biomac.9b00030
Keywords
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Funding
- Ministry of Science and Higher Education in Poland [7150/E -338/M/2017]
- National Science Centre of Poland [UMO-2016/21/D/ST5/01633]
- European Regional Development Fund [POIG.02.01.00-12-023/08]
- European Regional Development Fund Operational Program on Infrastructure and Environment [POIS 13.01.00-00-062/08]
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Poly(n-butyl methacrylate) (PBMA) or poly(n-butyl acrylate) (PBA)-grafted brush coatings attached to glass were successfully prepared using atom-transfer radical polymerization from the surface. The thicknesses and composition of the PBMA and PBA coatings were examined using ellipsometry and time-of-flight secondary ion mass spectrometry (ToF-SIMS), respectively. For PBMA, the glass-transition temperature constitutes a range close to the physiological limit, which is in contrast to PBA, where the glass-transition temperature is around -55 degrees C. Atomic force microscopy studies at different temperatures suggest a strong morphological transformation for PBMA coatings, in contrast to PBA, where such essential changes in the surface morphology are absent. Besides, for PBMA coatings, protein adsorption depicts a strong temperature dependence. The combination of bovine serum albumin and anti-IgG structure analysis with the principal component analysis of ToF-SIMS spectra revealed a different orientation of proteins adsorbed to PBMA coatings at different temperatures. In addition, the biological activity of anti-IgG molecules adsorbed at different temperatures was evaluated through tracing the specific binding with goat IgG.
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