Journal
BIOELECTROCHEMISTRY
Volume 129, Issue -, Pages 124-134Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2019.05.011
Keywords
Electrochemical sensors; Total antioxidant capacity; Water-soluble extracts; Gold nanoparticles
Funding
- Romanian National Authority for Scientific Research and Innovation, CNCS-UEFISCDI [PN-II-RU-TE-2014-4-2801]
- Romanian Ministry of Research and Innovation, CCCDI -UEFISCDI, within PNCDI III [PN-III-P1-1.2-PCCDI-2017-0062, 58]
- project PRO-DD (POS-CCE) [0.2.2.1., 123, SMIS 2637, 11/2009]
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The antioxidant properties of different plant extracts are usually claimed and used by food, medicine and cosmetic industry due to their health promoting capacities. In this study the presence of antioxidant compounds and the total antioxidant capacity of water-soluble extracts, prepared using two extraction methods and a variety of solvents, have been determined and a rapid screening method has been developed. Plant extracts characterisation, composition and antioxidant properties were confirmed by FTIR and Raman spectroscopies. Voltammetry, amperometry and electrochemical impedance were used to highlight the total antioxidant capacity of each extract using an electrochemical gold nanozyme-sensor based on the enzyme-like catalytic activity of gold nano particles. Both anodic area of cyclic voltammograms (10.31 mu A V) and electrochemical index (153) calculated using differential potential voltammetry show the total content of antioxidant compounds, allowing to discriminate between different extracts. Amperometric total antioxidant capacity measurements were associated with those from classical chemiluminescence and good correlation has been found (Pearson's correlation coefficient of 0.958). (C) 2019 Published by Elsevier B.V.
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