Journal
BIOELECTROCHEMISTRY
Volume 126, Issue -, Pages 29-37Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2018.11.010
Keywords
Droplet; Electroporation; Optimization; Transformation; Chlamydomonas reinhardtii
Funding
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2018R1D1A3B07047852]
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We performed the transformation of a wild type Chlamydomonas reinhardtii by optimizing previously developed droplet EP method. For more effective and faster optimization, we used DNA dying fluorescent molecule (Yo-Pro-1) for finding optimal EP conditions instead of using protein expression based evaluation method. By examining wider range of electrical parameter space together with the analysis of total current flow of EP process, we found optimal EP conditions. The obtained optimal EP conditions were verified by CFP transgene expression experiments. By applying the optimal EP conditions to the transformation of C. reinhardtii, we obtained transformants and analyzed them using PCR. Finally, implications and future work are discussed. (C) 2018 Elsevier B.V. All rights reserved.
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