Journal
AUTOPHAGY
Volume 16, Issue 2, Pages 313-333Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15548627.2019.1607694
Keywords
Autophagy; cell death; metabolism; redox system; stem cells differentiation
Categories
Funding
- European Regional Development Fund [POCI-01-0145-FEDER-007440, POCI-01-0145-FEDER-016390:CANCEL STEM]
- Fundacao para a Ciencia e a Tecnologia [IF/01316/2014]
- Instituto de Salud Carlos III [PI17/02009, FI14/00405]
- Instituto de Investigacion Sanitaria del Principado de Asturias
Ask authors/readers for more resources
Given the relatively long life of stem cells (SCs), efficient mechanisms of quality control to balance cell survival and resistance to external and internal stress are required. Our objective was to test the relevance of cell quality control mechanisms for SCs maintenance, differentiation and resistance to cell death. We compared cell quality control in P19 stem cells (P19SCs) before and after differentiation (P19dCs). Differentiation of P19SCs resulted in alterations in parameters involved in cell survival and protein homeostasis, including the redox system, cardiolipin and lipid profiles, unfolded protein response, ubiquitin-proteasome and lysosomal systems, and signaling pathways controlling cell growth. In addition, P19SCs pluripotency was correlated with stronger antioxidant protection, modulation of apoptosis, and activation of macroautophagy, which all contributed to preserve SCs quality by increasing the threshold for cell death activation. Furthermore, our findings identify critical roles for the PI3K-AKT-MTOR pathway, as well as autophagic flux and apoptosis regulation in the maintenance of P19SCs pluripotency and differentiation potential.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available