Journal
AQUACULTURE RESEARCH
Volume 50, Issue 6, Pages 1668-1677Publisher
WILEY
DOI: 10.1111/are.14049
Keywords
chemical analysis; fat; green lipped mussels; king salmon; near infrared reflectance spectroscopy
Categories
Funding
- New Zealand High Value Nutrition National Science Challenge [UOAX1421]
- Ministry of Business, Innovation and Employment [CAWX1606]
- New Zealand Ministry of Business, Innovation & Employment (MBIE) [CAWX1606, UOAX1421] Funding Source: New Zealand Ministry of Business, Innovation & Employment (MBIE)
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Greenshell (TM) mussel (GSM, Perna canaliculus) and king (Chinook) salmon (Oncorhynchus tshawytscha) are New Zealand's two major aquaculture species generating $380 million NZD in exports during the 2017-18 financial year. This study addresses the development and validation of a method based on Fourier transform-near infrared reflectance spectroscopy (FT-NIRs) to determine proximate composition for both species to aid breeding-, production- and consumer decisions. Rapid measurements of GSM (n = 176) were taken by FT-NIRs and analysed by traditional wet chemistry 'reference methods' to develop calibration models for proximate composition (protein, moisture, fat, ash and carbohydrate). The predictive models for moisture (r(2) = 0.98, root mean square error of cross validation (RMSECV) = 0.314, residual prediction deviation (RPD = 6.47), protein (r(2) = 0.91, RMSECV = 0.295, RPD = 3.01)) and carbohydrate (r(2) = 0.87, RMSECV = 0.440, RPD = 2.78) in GSM performed well. Additional models based on 90 portions of salmon were developed to predict moisture (r(2) = 0.98, RMSECV = 1.02, RPD = 7), protein (r(2) = 0.96, RMSECV = 0.347, RPD = 5.08), fat (r(2) = 0.99, RMSECV = 1.09, RPD = 5.98) and ash (r(2) = 0.72, RMSECV = 0.05, RPD = 1.9). The predictive FT-NIRs and reference methods were tested for short-term and intermediate precision, which demonstrated that the repeatability of the predictive models was comparable to the reference methods. Proximate analysis of GSM and king salmon using FT-NIRs was quick (minutes for sample preparation and analysis rather than days) and all components were assessed simultaneously. This provides a low-cost short turn-around method suitable for industry and research applications.
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