Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 58, Issue 27, Pages 9262-9268Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201904208
Keywords
axon transport; dynein; single-particle resolution; upconversion microscopy; wide-field illumination
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Funding
- Singapore Ministry of Education [R143000627112, R143000642112]
- Singapore National Research Foundation
- National Natural Science Foundation of China [21771135, 21701119]
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Cargo transport along axons, a physiological process mediated by motor proteins, is essential for neuronal function and survival. A current limitation in the study of axonal transport is the lack of a robust imaging technique with a high spatiotemporal resolution to visualize and quantify the movement of motor proteins in real-time and in different depth planes. Herein, we present a dynamic imaging technique that fully exploits the characteristics of upconversion nanoparticles. This technique can be used as a microscopic probe for the quantitative in situ tracking of retrograde transport neurons with single-particle resolution in multilayered cultures. This study may provide a powerful tool to reveal dynamic neuronal activity and intra-axonal transport function as well as any associated neurodegenerative diseases resulting from mutation or impairment in the axonal transport machinery.
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