Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 58, Issue 24, Pages 8013-8017Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201901192
Keywords
aptamers; biosensors; DNA; glutamate dehydrogenase; rolling circle amplification
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Funding
- National Science Foundation of China [21777013]
- Chinese Thousand Talents Program
- Fundamental Research Funds for the Central Universities [DUT17RC(3) 040]
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Canadian Foundation for Innovation (CFI)
- Ontario Ministry for Research and Innovation (ORF-RE) [RE07-045]
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We report on the first effort to select DNA aptamers from a circular DNA library, which resulted in the discovery of two high-affinity circular DNA aptamers that recognize the glutamate dehydrogenase (GDH) from Clostridium difficile, an established antigen for diagnosing Clostridium difficile infection (CDI). One aptamer binds effectively in both the circular and linear forms, the other is functional only in the circular configuration. Interestingly, these two aptamers recognize different epitopes on GDH, demonstrating the advantage of selecting aptamers from circular DNA libraries. A sensitive diagnostic test was developed to take advantage of the high stability of circular DNA aptamers in biological samples and their compatibility with rolling circle amplification. This test is capable of identifying patients with active CDI using stool samples. This work represents a significant step forward towards demonstrating the practical utility of DNA aptamers in clinical diagnosis.
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