Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 58, Issue 23, Pages 7713-7717Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201902347
Keywords
endocytosis; green fluorescent protein; halogens; membrane transport; tRNA synthetase
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Funding
- Science and Engineering Research Board (SERB), New Delhi
- SERB [SB/S2/JCB-067/2015]
- IISc, Bangalore
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Direct delivery of proteins into mammalian cells is a challenging problem in biological and biomedical applications. The most common strategies for the delivery of proteins into the cells include the use of cell-penetrating peptides or supercharged proteins. Herein, we show for the first time that a single atom change, hydrogen to halogen, at one of the tyrosine residues can increase the cellular entry of similar to 28kDa green fluorescent protein (GFP) in mammalian cells. The protein uptake is facilitated by a receptor-mediated endocytosis and the cargo can be released effectively into cytosol by co-treatment with the endosomolytic peptide ppTG21.
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