Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 58, Issue 27, Pages 9120-9124Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201903756
Keywords
coacervates; enzymes; membranes; proteinosomes; self-assembly
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Funding
- ERC Advanced Grant Scheme [740235]
- EPSRC [EP/L002957/1]
- BBSRC (BrisSynBio, Bristol Centre for Synthetic Biology) [BB/L01386X/1]
- European Research Council (ERC) [740235] Funding Source: European Research Council (ERC)
- BBSRC [BB/L01386X/1] Funding Source: UKRI
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The integration of molecularly crowded micro-environments into membrane-enclosed protocell models represents a step towards more realistic representations of cellular structure and organization. Herein, the membrane diffusion-mediated nucleation of either negatively or positively charged coacervate microdroplets within the aqueous lumen of individual proteinosomes is used to prepare nested hybrid proto-cells with spatially organized and chemically coupled enzyme activities. The location and reconfiguration of the entrapped droplets are regulated by tuning the electrostatic interactions between the encapsulated coacervate and surrounding negatively charged proteinosome membrane. As a consequence, alternative modes of a cascade reaction involving membrane- and coacervate-segregated enzymes can be implemented within the coacervate-in-proteinosome protocells.
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