Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 58, Issue 25, Pages 8474-8478Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201903165
Keywords
biocatalysis; kinetic resolution; photoenzymes; rational design; alpha-functionalized carboxylic acids
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Funding
- National Natural Science Foundation of China [21574113]
- Zhejiang Provincial Natural Science Foundation [LY19B020014]
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Chiral alpha-functionalized carboxylic acids are valuable precursors for a variety of medicines and natural products. Herein, we described an engineered fatty acid photodecarboxylase (CvFAP)-catalyzed kinetic resolution of alpha-amino acids and alpha-hydroxy acids, which provides the unreacted R-configured substrates with high yields and excellent stereoselectivity (ee up to 99%). This efficient light-driven process requires neither NADPH recycling nor prior preparation of esters, which were required in previous biocatalytic approaches. The structure-guided engineering strategy is based on the scanning of large amino acids at hotspots to narrow the substrate binding tunnel. To the best of our knowledge, this is the first example of asymmetric catalysis by an engineered CvFAP.
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