Journal
ANALYTICAL CHEMISTRY
Volume 91, Issue 11, Pages 7112-7117Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b00109
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Funding
- Shenzhen University [2017090]
- National Natural Science Foundation of China [31771036, 51703132]
- Basic Research Program of Shenzhen [JCYJ20180507182413022, JCYJ20170412111100742]
- Guangdong Province Natural Science Foundation of Major Basic Research and Cultivation Project [2018B030308003]
- Fok Ying-Tong Education Foundation for Young Teachers in the Higher Education Institutions of China [161032]
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Alkaline phosphatase (ALP) is distributed widely in living organisms and is an important biomarker closely related to many physiological and pathological processes. However, in vivo real-time detection of ALP remains a significant challenge. Herein, we developed a turn on molecular probe (denoted as LET-3) to visualize ALP activity in tumor tissues through near-infrared fluorescence (NIRF) and photoacoustic (PA) dual-modal imaging. LET-3, composed of NIR hemicyanine dye (LET-CyOH) and a phosphate moiety, showed a 23-fold NIRF enhancement at 730 nm and 27-fold PA enhancement at 710 nm upon activation by ALP. More importantly, both in vitro and in vivo diagnostic experiments indicated that LET-3 has a high sensitivity and good selectivity for ALP. These findings provide a promising strategy for in vivo ALP detection using NIRF and PA dual-channel turn-on probes.
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