Journal
ANALYTICA CHIMICA ACTA
Volume 1074, Issue -, Pages 123-130Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2019.05.008
Keywords
Fluorescent probe; Biothiols; Discriminative detection; Dual channels imaging
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Funding
- National Natural Science Foundation of China [21372063]
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Abnormal levels of Cys, Hcy and GSH are associated with various diseases, thus monitoring biothiols is of great significance. In this work, a dual-emission responsive near-infrared fluorescent probe NIR-NBD for detecting Hcy and Cys/GSH was developed based on the conjugation of a dicyanoisophorone based fluorophore (NIR-OH) and 7-nitrobenzofurazan (NBD). To our surprise, the addition of Hcy induced significant fluorescence enhancement at both 549 and 697 nm; while Cys/GSH resulted in major fluorescence emission at 697 nm. The detection limit was determined to be 33.2 nM for Cys, 33.5 nM for Hcy, and 34.4 nM for GSH. Therefore, the probe can be used for discriminative detection of Hcy and Cys/GSH. Moreover, fluorescence imaging of HeLa cells indicated that the probe was cell membrane permeable and could be used for visualizing Hcy and Cys/GSH in living cells. (C) 2019 Published by Elsevier B.V.
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