Journal
ALIMENTARY PHARMACOLOGY & THERAPEUTICS
Volume 49, Issue 11, Pages 1401-1409Publisher
WILEY
DOI: 10.1111/apt.15250
Keywords
-
Funding
- AbbVie
- Mundipharma
- Pfizer
- Janssen
- Atlantic Healthcare Limited
- Amgen
- Genentech
- Gilead Sciences
- Takeda
- Lilly
- Celgene/Receptos
- AbbVie Inc.
- Amgen Inc.
- AstraZeneca/MedImmune Ltd.
- Atlantic Pharmaceuticals Ltd.
- Boehringer-Ingelheim
- Celgene Corporation
- Celltech
- Genentech Inc/Hoffmann-La Roche Ltd.
- Gilead Sciences Inc.
- GlaxoSmithKline (GSK)
- Janssen Research & Development LLC.
- Pfizer Inc.
- Receptos Inc./Celgene International
- Sanofi
- Santarus Inc.
- Takeda Development Center Americas Inc.
- Tillotts Pharma AG
- UCB
Ask authors/readers for more resources
Background The appropriate location for biopsy procurement relative to an ulcer in active Crohn's disease is unknown. Aim To explore the relationship between biopsy location, histological disease activity, proinflammatory gene expression and the presence of inflammatory cells. Methods Fifty-one patients with Crohn's disease and ulcers >0.5 cm diameter in the colon and/or ileum were prospectively enrolled at three centres. Biopsies were obtained from 0 mm, 7 to 8 mm and 21 to 24 mm from the edge of the largest ulcer. Histological activity was blindly assessed with the Global Histological Disease Activity Score, the Robarts Histopathology and Nancy Histological indices. Messenger ribonucleic acid (mRNA) levels for interleukins-6, -8 and -23 (p19 and p40 subunits), CD31 and S100A9 were measured using quantitative polymerase chain reaction. The number of CD3+, CD68+ and myeloperoxidase-positive cells was quantified by immunohistochemistry. Data were analysed using mixed models with location and segment as fixed effects and patients as random effect to account for correlation among segments within a patient. Results Histological disease activity scores (P < 0.0001), proinflammatory gene expression levels (P < 0.005) and numbers of myeloperoxidase-positive cells (P < 0.0001) were highest in biopsies from the ulcer edge in the colon and ileum, with decreasing gradients observed with distance from the edge (P < 0.05). No differences between colonic and ileal samples were detected for the parameters measured at any location. Conclusions Biopsies from the ulcer edge in patients with Crohn's disease yielded the greatest histological disease activity and mRNA levels and had similar readouts in the colon and ileum. Research is needed to confirm this conclusion for other measures.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available