Journal
ADSORPTION-JOURNAL OF THE INTERNATIONAL ADSORPTION SOCIETY
Volume 25, Issue 4, Pages 855-864Publisher
SPRINGER
DOI: 10.1007/s10450-019-00085-7
Keywords
Zirconia/silica oxide materials; Aminoacylase from Aspergillus melleus; Immobilization process; Biocatalytic systems; Catalytic properties
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Funding
- Polish Ministry of Science and Higher Education [03/32/SBAD/0906]
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Oxide material of a new type, ZrO2 center dot SiO2 (raw and carbonyl-grafted), was used as support in the immobilization of aminoacylase from Aspergillus melleus. The ZrO2 center dot SiO2 was synthesized via sol-gel method. The obtained material was additionally modified with glutaraldehyde. Various physicochemical analyses were used to confirm the effectiveness of the modification and immobilization processes, including Fourier transform infrared spectroscopy, laser Doppler velocimetry and low-temperature N-2 sorption. The immobilization process was performed within 3 h using different concentrations of enzyme solution (1, 3, 5 and 7 mg/mL), and the Bradford method was used to determine the quantity of immobilized enzyme. The resulting biocatalytic systems were then used as catalysts in the hydrolysis of different N-acetyl-DL-amino acids (leading to L-methionine, L-cysteine, L-serine and L-tryptophan). Based on this reaction the apparent and relative catalytic activities were determined. The highest activity of the immobilized enzyme was attained in the synthesis of l-methionine (the apparent activities of aminoacylase immobilized on raw and carbonyl-grafted ZrO2 center dot SiO2 were 4112 and 4947 U/g, respectively). Furthermore, the effect of pH and temperature on catalytic activity, as well as the storage stability and reusability of the prepared biocatalytic systems were determined. Aminoacylase immobilized on carbonyl-grafted ZrO2 center dot SiO2 retains 85% of its initial activity after 30 days of storage and 70% after five reaction cycles.
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