Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 11, Issue 22, Pages 19724-19733Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b02107
Keywords
spherical nucleic acids; DNA circuit; visible disassembly; DNA-strand-displacement; single nucleotide polymorphism discrimination; colorimetric detection
Funding
- National Natural Science Foundation of China [91427304, 21434007, 21504021, 21574122, 31501576, 51573175]
- Fundamental Research Funds for the Central Universities [WK3450000002, WK2060200026]
- China Postdoctoral Science Foundation [2018M630708]
- National Postdoctoral Program for Innovative Talents [BX20180285]
- Excellent Talent Foundation of Education Department of Anhui Province [gxyq2019066]
- 136 talent plan of Hefei Normal University
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The programmable toehold-mediated DNA-strand-displacement reaction has demonstrated its extraordinary capability in driving the spherical nucleic acid assembly. Here, a facile strategy of integrating a DNA-strand-displacement-based DNA circuit with a universal spherical nucleic acid aggregate system was developed for the visible disassembly of spherical nucleic acids. This integrated system exhibited rapid colorimetric response and good sensitivity in the disassembly reaction and demonstrated its capability in the application of single nucleotide polymorphism discrimination. Moreover, an OR logic gate used for multiplex detection was constructed through combining the fixed spherical nucleic acid disassembly system with two DNA circuits. This strategy will have great potential in the fabrication of a portable low-cost DNA diagnostic kit, and it is also a very promising method to be used in other applications, such as complex DNA networks and programmable phase transformation of nanoparticle superlattices.
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