Journal
MITOCHONDRIAL DNA PART B-RESOURCES
Volume 4, Issue 1, Pages 413-415Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/23802359.2018.1547158
Keywords
Illumina sequencing; mitogenomics; inquiry-based learning; Trichoptera; Limnephilidae
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Funding
- University of Manitoba Research Grants Program
- Faculty of Science Field Work Support Program
- CATL Teaching and Learning Enhancement Fund
- NSERC [RGPIN386337-2011, RGPIN-2016-06012]
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The pale summer sedge caddisfly, Limnephilus hyalinus Hagen, 1861 (Limnephilidae, the Northern Caddisflies), is widespread in North America. Genome skimming by Illumina sequencing allowed assembly of a complete 15,168 bp circular mitogenome from L. hyalinus consisting of 78.0% AT nucleotides, 22 tRNAs, 13 protein-coding genes, two rRNAs and a control region in the ancestral insect gene order. Limnephilus hyalinus COX1 features an atypical CGA start codon while ATP8, NAD1, NAD5, and NAD6 exhibit incomplete stop codons. The mtTERM binding site is conserved between the Trichoptera and the Lepidoptera. Phylogenetic reconstruction reveals a monophyletic Order Trichoptera, Family Limnephilidae, and genus Limnephilus.
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