4.6 Article

De novo transcriptome assembly and analysis of Sf21 cells using illumina paired end sequencing

Journal

BIOLOGY DIRECT
Volume 10, Issue -, Pages -

Publisher

BMC
DOI: 10.1186/s13062-015-0072-7

Keywords

Army worm; Insect; Pest; Sf21 cells; Spodoptera frugiperda

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Funding

  1. Dept. of Biotechnology, Govt of India [BT/PR10673/AGR/36/579/2008]

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Spodoptera is an important polyphagous agricultural insect pest in the tropical world. The genomic details are limited to understand the pest biology at molecular level. In the present study, we sequenced and assembled the transcriptome from Sf21 cells into a non redundant set of 24,038 contigs of similar to 47.38 Mb in size. A total of 26,390 unigenes were identified from the assembled transcripts and their annotation revealed the prevalent protein domains in Sf21 cells. The present study would provide a resource for gene discovery and development of functional molecular markers to understand the biology of S. frugiperda.

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