Journal
EBIOMEDICINE
Volume 42, Issue -, Pages 504-510Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ebiom.2019.03.017
Keywords
Iron deficiency; Anaemia; Lateral flow immunoassay; Soluble transferrin receptor; Point-of-care testing; Portable diagnostics
Funding
- Thrasher Research Fund Early Career Award [13379]
- National Institutes of Health (NIH) [NIH R03 EB 023190]
- National Science Foundation (NSF) [1343058]
- Nutrition International, formerly Micronutrient Initiative [10-8007-CORNE-01]
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Background: Iron deficiency (ID) and anaemia are major health concerns, particularly in young children. Screening for ID based on haemoglobin (Hb) concentration alone has been shown to lack sensitivity and specificity. The American Academy of Pediatrics (AAP) recommends soluble transferrin receptor (sTfR) as a promising approach to screen for iron deficiency. However, in most settings, assessment of iron status requires access to centralized laboratories. There is an urgent need for rapid, sensitive, and affordable diagnostics for sTfR at the point-of-care. Methods: An immunochromatographic assay-based point-of-care screening device was developed for rapid quantification of sTfR from a drop of serum within a few minutes. Performance optimization of the assay was done in sTfR-spiked buffer and commercially available sTfR calibrator, followed by a small-scale proof-of-concept validation with archived serum samples. Findings: On preliminary testing with archived serum samples and comparison with Ramco ELISA, a correlation of 0.93 (P < 0.0001) was observed, demonstrating its potential for point-of-care assessment of iron status. Interpretation: The analytical performance of the point-of-care sTfR screening device indicates the potential for application in home-use test kits and field settings, especially in low-andmiddle-income settings. An added advantage of sTfR quantification in combination with our previously reported serum ferritin diagnostics is in integration of Cook's equation as a quantitative and minimally-invasive indicator of total body iron stores. (C) 2019 The Authors. Published by Elsevier B.V.
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