Journal
CHEM
Volume 5, Issue 4, Pages 848-857Publisher
CELL PRESS
DOI: 10.1016/j.chempr.2019.01.001
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Funding
- National Natural Science Foundation of China [21750005, 31470532, 91543102, 21532004, 21621003, 31711530153]
- Ministry of Science and Technology of China [2015CB553402, 2016YFC0206300, 2017YFA0505200]
- Beijing Nova Program [Z171100001117011]
- Tsinghua University Initiative Scientific Research Program [20161080152]
- China Postdoctoral Science Foundation [2017M620751]
- Tsinghua University-Peking University Center for Life Sciences (CLS)
- Beijing Frontier Research Center for Biological Structure
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The flow of genetic information between DNA, RNA, and protein lays themolecular foundation of life. Here, we show the transcription of a mirror-image gene into L-RNA and reverse transcription into L-DNA by synthetic D-polymerases based on designed mutants of the DNA polymerase Dpo4. Furthermore, the reverse-transcribed L-DNA was amplified by mirror-image PCR and sequenced by nucleobase-specific chemical cleavage. As a step toward the synthesis of amirror-image ribosome, we enzymatically transcribed full-length mirror-image 5S rRNA. The realization of a comprehensive mirror-image transcription, reverse transcription, PCR, and sequencing toolbox will enable the directed evolution of nuclease-resistant L-RNA aptamers and ribozymes and spur the development of other functional mirror-image molecular systems.
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