Journal
HLA
Volume 93, Issue 6, Pages 474-483Publisher
WILEY
DOI: 10.1111/tan.13535
Keywords
DNA; genetic; hematopoietic stem cell transplantation; histocompatibility testing; human leukocyte antigens; polymorphism; sequence analysis
Categories
Funding
- Office of Naval Research [N00014-16-1-3133]
Ask authors/readers for more resources
Next generation DNA sequencing is used to determine the HLA-A, -B, -C, -DRB1, -DRB3/4/5, and -DQB1 assignments of 1009 unrelated volunteers for the unrelated donor registry in The Netherlands. The analysis characterizes all HLA exons and introns for class I alleles; at least exons 2 to 3 for HLA-DRB1; and exons 2 to 6 for HLA-DQB1. Of the distinct alleles present, there are 229 class I and 71 class II; 36 of these alleles are novel. The majority ( approximately 98%) of the cumulative allele frequency at each locus is contributed by alleles that appear three or more times. Alleles encoding protein variation outside of the antigen recognition domains are 0.6% of the class I assignments and 5.3% of the class II assignments.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available