Journal
ACS SENSORS
Volume 4, Issue 4, Pages 832-840Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.8b01188
Keywords
optofluidics; random laser; cytometer; phenotype; cell suspension; biophysics; cancer cells
Funding
- Central Research Fund Project [Poly U 152207/15E]
- PolyU Central Grant project [1-ZVGB]
- National Natural Science Foundation of China [31500758]
- Croucher Foundation
- City University of Hong Kong [7004602]
- Hong Kong Research Grant Council [C1013-15GF]
- H2020 Marie Sklodowska-Curie Actions (MSCA) [744817]
- China Postdoctoral Science Foundation [2015M571918, 2017T100442]
- [11206014]
- Marie Curie Actions (MSCA) [744817] Funding Source: Marie Curie Actions (MSCA)
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Phenotypic profiling of single floating cells in liquid biopsies is the key to the era of precision medicine. A random laser in biofluids is a promising tool for the label-free characterization of the biophysical properties as a result of the high brightness and sharp peaks of the lasing spectra, yet previous reports were limited to the random laser in solid tissues with dense scattering. In this report, a random laser cytometer is demonstrated in an optofluidic device filled with gain medium and human breast normal/cancerous cells. The multiple lightscattering event induced by the microscale human cells promotes random lasing and influences the lasing properties in term of laser modes, spectral wavelengths, and lasing thresholds. A sensing strategy based on analyzing the lasing properties is developed to determine both the whole cell and the subcellular biophysical properties, and the malignant alterations of the cell suspensions are successfully detected. Our results provide a new approach to designing a label-free biophysical cytometer based on optofluidic random laser devices, which is advantageous for further research in the field of random laser bioapplication.
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