Journal
ACS SENSORS
Volume 4, Issue 3, Pages 562-565Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.8b01134
Keywords
acetylation; FLIM; real-time detection; peptide sensor; live cultures
Funding
- W. M. Keck Foundation
- Purdue University Center for Cancer Research, NIH [P30CA023168]
- Indiana CTSI Grant from NIH [358 UL1TR001108]
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Acetyltransferase is a member of the transferase group responsible for transferring an acetyl group from acetyl-CoA to amino group of a histone lysine residue. Past efforts on histone acetylation monitoring involved biochemical analysis that do not provide spatiotemporal information in a dynamic format. We propose a novel approach to monitor acetyltransferase acetylation in live single cells using time correlated single photon counting fluorescence lifetime imaging (TCSPC-FLIM) with peptide bio-sensors. Utilizing 2D and 3D cultures we show that the peptide sensor has a specific response to acetyltransferase enzyme activity in a fluorescence lifetime dependent manner (P < 0.001). Our FLIM biosensor concept enables real-time longitudinal measurement of acetylation activity with high spatial and temporal resolution in live single cells to monitor cell function or evaluate drug effects to treat cancer or neurological diseases.
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