Journal
ACS SENSORS
Volume 4, Issue 5, Pages 1185-1189Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.9b00228
Keywords
solid-state nanopore; single protein detection; cross-linker; dielectric breakdown; functionalization
Funding
- National Natural Science Foundation of China [61871183, 21834001]
- Ten Thousand Talent Program for young top-notch talent, Innovation Program of Shanghai Municipal Education Commission [2017-01-07-00-02-E00023]
- Shanghai Science and Technology Development Fund [19QA1402300]
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Single protein sensing based on solid-state nanopores is promising but challenging, because the fast translocation velocity of a protein is beyond the bandwidth of nanopore instruments. To decelerate the translocation speed, here, we employed a common protein cross-link interaction to achieve a general and robust nanopore sensing platform for single-molecule detection of protein. Benefiting from the EDC/NHS coupling interaction between nanopore and proteins, a 10-fold decrease in speed has been achieved. The clearly distinguishable current signatures further reveal that the anisotropic translocation of a protein, which are horizontal, vertical, and flipping transit inside nanopore confinement. This strategy provides a general platform for rapid detection of proteins as well as exploring fundamental protein dynamics at the single-molecule level.
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